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THE
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IMMUNOGENETICS
INFORMATION SYSTEM®

WELCOME !


Citing IMGT/V-QUEST:
Brochet, X. et al., Nucl. Acids Res. 36, W503-508 (2008). PMID: 18503082
Giudicelli, V., Brochet, X., Lefranc, M.-P., Cold Spring Harb Protoc. 2011 Jun 1;2011(6). pii: pdb.prot5633. doi: 10.1101/pdb.prot5633.
PMID: 21632778 Abstract also in IMGT booklet with generous provision from Cold Spring Harbor (CSH) Protocols (high res) (lower res)

IMGT/V-QUEST programme version: 3.2.30 (28 January 2013) - IMGT/V-QUEST reference directory release: 201310-4 (14 March 2013)

Analyse your IG or antibody nucleotide sequences

Your selection: Channel catfish

Your sequences will be compared to the Channel catfish (Ictalurus punctatus) IG set from the IMGT/V-QUEST reference directory sets

Sequence submission

Type (or copy/paste) your nucleotide sequence(s) in FASTA format

Or give the path access to a local file containing your sequence(s) in FASTA format

Display results

A. Detailed view HTML Text Nb of nucleotides per line in alignments: Nb of aligned reference sequences:
1. Alignment for V-GENE
2. Alignment for D-GENE
3. Alignment for J-GENE
4. Results of IMGT/JunctionAnalysis
     with full list of eligible D-GENE
     without list of eligible D-GENE
5. Sequence of the JUNCTION ('nt' and 'AA')
  6. V-REGION alignment
  7. V-REGION translation
  8. V-REGION protein display
  9. V-REGION mutation and AA change table
10. V-REGION mutation and AA change statistics
11. V-REGION mutation hot spots
12. Sequences of V-, V-J- or V-D-J- REGION ('nt' and 'AA') with gaps in FASTA
               and access to IMGT/PhyloGene for V-REGION ('nt')
13. Annotation by IMGT/Automat

14. IMGT Collier de Perles
       link to IMGT/Collier-de-Perles tool
       IMGT Collier de Perles (for a nb of sequences < 5)
       no IMGT Collier de Perles

B. Synthesis view HTML Text Nb of nucleotides per line in alignments: Summary table sequence order
1. Alignment for V-GENE
2. V-REGION alignment
3. V-REGION translation
4. V-REGION protein display
  5. V-REGION protein display (with AA class colors)
  6. V-REGION protein display (only AA changes displayed)
  7. V-REGION most frequently occurring AA
  8. Results of IMGT/JunctionAnalysis

C. Excel file
1. Summary
2. IMGT-gapped-nt-sequences
3. nt-sequences
4. IMGT-gapped-AA-sequences
5. AA-sequences
6. Junction
  7. V-REGION-mutation-and-AA-change-table
  8. V-REGION-nt-mutation-statistics
  9. V-REGION-AA-change-statistics
10. V-REGION-mutation-hot-spots
11. Parameters

Advanced parameters

Selection of IMGT reference directory set With all alleles With allele *01 only
Search for insertions and deletions in V-REGION Yes (slower, the nb of submitted sequences in a single run is limited to 10) No
Parameters for IMGT/JunctionAnalysis Nb of accepted D-GENE in IGH JUNCTION (default is 1) Nb of accepted mutations: in 3'V-REGION
in D-REGION
in 5'J-REGION

Parameters for "Detailed view" Nb of nucleotides to exclude in 5' of the V-REGION for the evaluation of the nb of mutations (in results 9 and 10)
Nb of nucleotides to add (or exclude) in 3' of the V-REGION for the evaluation of the alignment score (in results 1)


Contact IMGT/V-QUEST Montpellier, France:
Véronique Giudicelli (Veronique.Giudicelli@igh.cnrs.fr) and Marie-Paule Lefranc (Marie-Paule.Lefranc@igh.cnrs.fr)


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