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IMGT Aide-mémoire

Genetic code

Nucleotide position in codon
first second third
U C A G
U UUU Phe
UUC Phe
UUA Leu
UUG Leu
UCU Ser
UCC Ser
UCA Ser
UCG Ser
UAU Tyr
UAC Tyr
UAA Stop
UAG Stop
UGU Cys
UGC Cys
UGA Stop
UGG Trp
U
C
A
G
C CUU Leu
CUC Leu
CUA Leu
CUG Leu
CCU Pro
CCC Pro
CCA Pro
CCG Pro
CAU His
CAC His
CAA Gln
CAG Gln
CGU Arg
CGC Arg
CGA Arg
CGG Arg
U
C
A
G
A AUU Ile
AUC Ile
AUA Ile
AUG Met
ACU Thr
ACC Thr
ACA Thr
ACG Thr
AAU Asn
AAC Asn
AAA Lys
AAG Lys
AGU Ser
AGC Ser
AGA Arg
AGG Arg
U
C
A
G
G GUU Val
GUC Val
GUA Val
GUG Val
GCU Ala
GCC Ala
GCA Ala
GCG Ala
GAU Asp
GAC Asp
GAA Glu
GAG Glu
GGU Gly
GGC Gly
GGA Gly
GGG Gly
U
C
A
G
Amino acids and codons
Amino acids Codons
A Ala Alanine GCA GCC GCG GCU
C Cys Cysteine UGC UGU
D Asp Aspartic acid GAC GAU
E Glu Glutamic acid GAA GAG
F Phe Phenylalanine UUC UUU
G Gly Glycine GGA GGC GGG GGU
H His Histidine CAC CAU
I Ile Isoleucine AUA AUC AUU
K Lys Lysine AAA AAG
L Leu Leucine UUA UUG CUA CUC CUG CUU
M Met Methionine AUG
N Asn Asparagine AAC AAU
P Pro Proline CCA CCC CCG CCU
Q Gln Glutamine CAA CAG
R Arg Arginine AGA AGG CGA CGC CGG CGU
S Ser Serine AGC AGU UCA UCC UCG UCU
T Thr Threonine ACA ACC ACG ACU
V Val Valine GUA GUC GUG GUU
W Trp Tryptophan UGG
Y Tyr Tyrosine UAC UAU
Stop codons UAA UGA UAG

The Genetic Code

The universal genetic code includes 20 common amino acids. In addition, selenocysteine (Sec) and pyrrolysine (Pyl), known as the twenty first and twenty second amino acids, are encoded by UGA and UAG, respectively, which are the codons that usually function as stop signals. Search for a putative twenty-third amino acid in archeal and bacterial genomes has been negative so far [1].

Restriction enzyme sites

Useful restriction sites
BamHI G↓GATCC
EcoRI G↓AATTC
HindIII A↓AGCTT
KpnI GGTAC↓C
PstI CTGCA↓G
PvuII CAG↓CTG
SacI (SstI) GAGCT↓C
TaqI T↓CGA
XbaI T↓CTAGA
XhoI C↓TCGAG

REBASE, The Restriction Enzyme Database, restriction enzymes and methylases.

Reference:
[1] Lobanov, A.V. et al., Trends in Genetics, 22, 357-360 (2006). PMID: 16713651 doi:10.1016/j.tig.2006.05.002