IMGT/HighV-QUEST (V-QUERy and STandardization) is part of IMGT®, the international ImMunoGeneTics information system® http://www.imgt.org .
IMGT/HighV-QUEST is the high-throughput version of IMGT/V-QUEST [3,4] for the analysis of thousands of immunoglobulin (IG) and T cell receptor (TR) rearranged nucleotide sequences per run.
IMGT/HighV-QUEST has been developed by IMGT®, the international ImMunoGeneTics Information System® to answer the problematic of the analysis of the antigen receptor data from Next-Generation Sequencing (NGS).
- All IMGT/HighV-QUEST users should be registered in order to be able to use this platform.
- IMGT/HighV-QUEST works with rearranged V-J and V-D-J sequences and germline V-GENEs, but does not work with germline D-GENEs or J-GENEs.
- IMGT/HighV-QUEST can optionally analyse sequences with DNA insertions or deletions (which do not respect the IMGT unique numbering).
- Sequences containing two V domains (as scFv) need to be split (at the level of the linker) prior to IMGT/HighV-QUEST analysis.
- IMGT/HighV-QUEST does not work with out-of-frame pseudogenes as they cannot be numbered according to the amino acid IMGT unique numbering. You may use BLAST in order to compare your sequence with F+ORF+all P genes and alleles IMGT reference sequences (select for "Database" : "IMGT/GENE-DB reference sequences").
- IMGT/HighV-QUEST does not work, or will give aberrant results, for too short partial sequences, sequences containing a cluster of V-GENEs, or sequences with too long 5'UTR or 3'UTR. For these sequences, you may use BLAST in order to identify homologous sequences against IMGT/LIGM-DB (select for "Database" : "IMGT/LIGM-DB") .