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IMGT/HighV-QUEST

IMGT/HighV-QUEST[1] (V-QUERy and STandardization) is part of IMGT®, the international ImMunoGeneTics information system® http://www.imgt.org [2].

IMGT/HighV-QUEST is the high-throughput version of IMGT/V-QUEST [3,4] for the analysis of thousands of immunoglobulin (IG) and T cell receptor (TR) rearranged nucleotide sequences (up to 150 000 sequences) per run.

IMGT/HighV-QUEST has been developed by IMGT®, the international ImMunoGeneTics Information System® to answer the problematic of the analysis of the antigen receptor data from Next-Generation Sequencing (NGS).

Note that:

• All IMGT/HighV-QUEST users should be registered in order to be able to use this platform.
• IMGT/HighV-QUEST works with rearranged V-J and V-D-J sequences and germline V-GENEs, but does not work with germline D-GENEs or J-GENEs.
• IMGT/HighV-QUEST can optionally analyse sequences with DNA insertions or deletions (which do not respect the IMGT unique numbering).
• Sequences containing two V domains (as scFv) need to be split (at the level of the linker) prior to IMGT/HighV-QUEST analysis.
• IMGT/HighV-QUEST does not work with out-of-frame pseudogenes as they cannot be numbered according to the amino acid IMGT unique numbering. You may use BLAST in order to compare your sequence with F+ORF+all P genes and alleles IMGT reference sequences (select for "Database" : "IMGT/GENE-DB reference sequences").
• IMGT/HighV-QUEST does not work, or will give aberrant results, for too short partial sequences, sequences containing a cluster of V-GENEs, or sequences with too long 5'UTR or 3'UTR. For these sequences, you may use BLAST in order to identify homologous sequences against IMGT/LIGM-DB (select for "Database" : "IMGT/LIGM-DB") .

Once registered and the submission is accepted by IMGT/HighV-QUEST administrator, users can log in into IMGT/HighV-QUEST using their e-mail and the password they selected during the registration. The first page that a user may see after logging in is the Search Page of IMGT/HighV-QUEST.

• IMGT/HighV-QUEST Search page
• IMGT/HighV-QUEST Documentation

Citing IMGT:
Lefranc, M.-P. et al., "IMGT®, the international ImMunoGeneTics database®" Nucl. Acids Res, 37, D1006-D1012 (2009); doi:10.1093/nar/gkn838. PMID: 18978023

Citing IMGT/HighV-QUEST:
Alamyar, E. et al., IMGT/HighV-QUEST: A High-Throughput System and Web Portal for the Analysis of Rearranged Nucleotide Sequences of Antigen Receptors, JOBIM 2010, Paper 63 (2010).
Brochet, X. et al., Nucl. Acids Res. 36, W503-508 (2008). PMID: 18503082

References:

[1]	Alamyar, E. et al., JOBIM2010, Paper 63 (2010).
[2]	Lefranc, M.-P. et al., "IMGT®, the international ImMunoGeneTics database®" Nucl. Acids Res, 37, D1006-D1012 (2009); doi:10.1093/nar/gkn838. PMID: 18978023
[3]	Giudicelli, V. et al., Nucl. Acids Res. 32, W435-440 (2004). PMID: 15215425, LIGM:287
[4]	Brochet, X. et al., Nucl. Acids Res. 36, W503-508 (2008). PMID: 18503082, LIGM:344

See also:

• Colliers de Perles
• IMGT
• IMGT/V-QUEST
• Numbering
• Tools

Created: 03/12/1999
Last updated: Wednesday, 29-Jun-2011 17:02:00 CEST
Authors: Véronique Giudicelli and Marie-Paule Lefranc
Editors: Manuel Ruiz and Chantal Ginestoux
Contact: Marie-Paule Lefranc

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Software material and data coming from IMGT server may be used for academic research only, provided that it is referred to IMGT®, and cited as "IMGT®, the international ImMunoGeneTics information system® http://www.imgt.org (founder and director: Marie-Paule Lefranc, Montpellier, France)." References to cite: Lefranc, M.-P. et al., Nucleic Acids Research, 27, 209-212 (1999) Cover of NAR; Ruiz, M. et al., Nucleic Acids Research, 28, 219-221 (2000); Lefranc, M.-P., Nucleic Acids Research, 29, 207-209 (2001); Lefranc, M.-P., Nucleic Acids Res., 31, 307-310 (2003); Lefranc, M.-P. et al., In Silico Biol., 5, 0006 (2004) [Epub], 5, 45-60 (2005); Lefranc, M.-P. et al., Nucleic Acids Res., 33, D593-D597 (2005) Full text; Lefranc, M.-P. et al., Nucleic Acids Research 2009 37: D1006-D1012; doi:10.1093/nar/gkn838 Full text.
For any other use please contact Marie-Paule Lefranc Marie-Paule.Lefranc@igh.cnrs.fr.

     

IMGT founder and director: Marie-Paule Lefranc Marie-Paule.Lefranc@igh.cnrs.fr
Bioinformatics manager: Véronique Giudicelli Veronique.Giudicelli@igh.cnrs.fr
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