A unique probe pH60 can detect all the TRG rearrangements whatever the TRGJ genes involved in the rearrangements (Huck, S. and Lefranc, M.-P., FEBS Lett., 224, 291-296 (1988)).
| Click here to see | Part 2 - Assignments of the TRG rearrangements to given J genes by hybridization of KpnI digests |
Since the TRGJ1 and TRGJ2 genes are highly homologous [3], it is possible, with the TRGJ1 probe, pH60 [1] [3], to detect the TRGV rearrangements to TRGJ1 and TRGJ2. Moreover, the sizes of the rearranged restriction fragments when pH60 is hybridized to BamHI, HindIII and EcoRI digests, allow allocation to a given TRGV of the rearragements observed in whole thymus, T cell lines or T cell leukemias [4]. This table shows the rearranged restriction fragments corresponding to the nine TRGV genes known to rearrange [2] [4].
Reference for this table: Foster, A. et al., EMBO J., 6, 1945-1950 (1987).
Probe: pH60 clone (TRGJ probe)[1] [3].
| TRG subgroup | TRG gene name |
BamHI | HindIII | EcoRI |
| TRGV1 | V2 | 11.7 | 4.3 | 0.9 |
| V3 | 16 | 3.7 | 5.4(+V2) | |
| V4 | 21 | 4.3 | 0.9 | |
| V5 | 25 | 3.6 | 2.2 | |
| V7 | 35 | 2.9 | 3.1 | |
| V8 | 40 | 2.9 | 4.2 | |
| TRGV2 | V9 | 15.5 | 4.0 | 2.4 |
| TRGV3 | V10 | 19 | 5.1 | 0.65 |
| TRGV4 | V11 | 12 | 5.6 | 9.5 |
References:
| [1] | Lefranc, M.-P. and Rabbitts, T.H., Nature, 316, 464-466 (1985). |
| [2] | Lefranc, M.-P. et al., Cell, 45, 237-246 (1986). |
| [3] | Lefranc, M.-P. et al., Nature, 319, 420-422 (1986). |
| [4] | Foster, A. et al., EMBO J., 6, 1945-1950 (1987). |
| [5] | Huck, S. and Lefranc, M.-P., FEBS Lett., 224, 291-296 (1988). |
Created: 12/05/1999
Authors: Violaine Moreau and Marie-Paule Lefranc
Contact: Marie-Paule Lefranc
Software material and data coming from IMGT server may be used for academic research only, provided that it is referred to IMGT®, and cited as "IMGT®, the international ImMunoGeneTics information system® http://www.imgt.org (founder and director: Marie-Paule Lefranc, Montpellier, France)." References to cite: Lefranc, M.-P. et al., Nucleic Acids Research, 27, 209-212 (1999) Cover of NAR; Ruiz, M. et al., Nucleic Acids Research, 28, 219-221 (2000); Lefranc, M.-P., Nucleic Acids Research, 29, 207-209 (2001); Lefranc, M.-P., Nucleic Acids Res., 31, 307-310 (2003); Lefranc, M.-P. et al., In Silico Biol., 5, 0006 (2004) [Epub], 5, 45-60 (2005); Lefranc, M.-P. et al., Nucleic Acids Res., 33, D593-D597 (2005) Full text; Lefranc, M.-P. et al., Nucleic Acids Research 2009 37: D1006-D1012; doi:10.1093/nar/gkn838 Full text.
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