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Database contains
1864 primers

IMGT/PRIMER-DB Primer card: IPP800004

PRIMER CATALOGUE
IMGT/PRIMER-DB Primer accession number IPP800004
IMGT/PRIMER-DB entry date 25/11/2003
IMGT/PRIMER-DB update 25/11/2003
IMGT/PRIMER-DB Primer definition Homo sapiens IGKV2-29*01 Sense primer Sense
Primer alias name 5'A183
Bibliographic references
(PubMed)		 PMID: 9234481
Catalogue comments IGKV2-29 gene specific
PRIMER CHARACTERISTICS
IMGT/PRIMER-DB Primer sequence 20 pb; 5 A; 4 C; 5 G; 6 T; 0 other

5' TAATTTCAGGATCCAGTGCG 3'
IMGT/PRIMER-DB Sequence length (number of nucleotides) 20
IMGT/PRIMER-DB Primer orientation Sense
IMGT/LIGM-DB reference sequence
Accession number		 X63396
IMGT/LIGM-DB reference sequence
Primer position (start-end)		 729-748
Primer Localization 
...CCTAAAAGCAGT   540
GCTCTGAATAATATCTTGAGAAATGAAAGAACTCTTGTGCCTATTTAATAAAGGGTTCAT   600
TTAAAATTTTGTTTTTATGATATGAATACAAATTTGTAAAAATAAAAGATTAGCCATAAA   660
TCAATATCATAAGGCAAATCTCAAAAGTTGTTCATTATGCTTTCACATAACCTTGCACTT   720
CTCTCTCATAATTTCAGGATCCAGTGCGGATATTGTGATGACCCAGACTCCACTCTCTCT   780
GTCCGTCACCCCTGGACAGCCGGCCTCCATCTCCTGCAAGTCTAGTCAGAGCCTCCTGCA   840
TAGTGATGGAAAGACCTATTTGTATTGGTACCTGCAGAAGCCAGGCCAGTCTCCACAGCT   900
CCTGATCTATGAAGTTTCCAGCCGGTTCTCTGGAGTGCCAGATAGGTTC...   1247
Characteristics comments IGKV2-29*01 Allele chosen as a representative of the IGKV2-29 Gene
IMGT/PRIMER-DB Set IPS000154
PRIMER CLASSIFICATION
IMGT/LIGM-DB reference sequence
Classification		 Species Homo sapiens
IMGT group IGKV
IMGT subgroup IGKV2
IMGT gene name IGKV2-29
IMGT allele name IGKV2-29*01
Classification comments and specificity The most 3' nucleotide G allows to specifically hybridize IGKV2-29 alleles
at the exception of the IGKV2-29*03 allele (L-PART2 g12>a) which has a A at position 12
as the IGKV2D-29 gene.
PRIMER DESCRIPTION
IMGT/PRIMER-DB Primer labels
PRIMER 1..20
V-INTRON 1..20
Differences between
the IMGT/LIGM-DB reference sequence
and the primer sequence		  
Restriction enzyme name  
Restriction site position  
Purification methods  
Keywords  
Description comments  
Created:
22/02/2002
Last updated:
21/11/2006
Developped and maintained:
Julien Bertrand (LIGM, Montpellier, France), Géraldine Folch (EUROGENTEC) and François Ehrenmann (LIGM, Montpellier, France).
Scientific responsibles:
Marc Lemaître (EUROGENTEC, Belgium) and Marie-Paule Lefranc (LIGM, France) Marie-Paule.Lefranc@igh.cnrs.fr