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Single chain Fragment variable (scFv)

Representation of a scFv with the IMGT Labels for the two V-DOMAIN.

The relative position 5' and 3' of the two V-DOMAIN in the scFv, in this figure, is arbitrary. At least 23 and 19 labels are necessary to describe a V-D-J-REGION and a V-J-REGION, respectively in IMGT/V-QUEST. The conserved 1st-CYS 23, CONSERVED-TRP 41, 2nd-CYS 104 and J-TRP or J-PHE 118 based on the IMGT unique numbering are shown in both V-DOMAIN [1-4].

[1]Lefranc M-P. Front Immunol. 2014;5:22. Open access PMID: 24600447 LIGM#429
[2] Lefranc M-P et al. Dev Comp Immunol. 2003;27:55-77. PMID:12477501
[3] Lefranc M-P. Cold Spring Harb Protoc. 2011;6:633-42.PMID: 21632789
[4] Lefranc M-P. Methods Mol Biol. 2014;1184:59-107. PMID: 25048119